PhD in Biochemistry & Molecular Biology
Work phone: (240)-784-3793
Email: louisethines@gmail.com
Education and Experience
Post-doctoral research fellow
National Institutes of Health, Clinical Center – September 2020 - Now
Role of calmodulin and IQGAPs in signal transduction pathways
Award: 2022 Fellows award for research excellence (FARE)
Research assistant
UCLouvain, Louvain Institute of Biomolecular Science & Technology – January - March 2020
Biochemical characterization of yeast proteins
PhD in biological engineering (FNRS fellow)
UCLouvain, Louvain Institute of Biomolecular Science & Technology – September 2015 - November 2019
PhD thesis ‘The yeast protein Gdt1 is a newly-identified actor in manganese homeostasis at the Golgi’
MSc in bioengineering sciences - Chemistry and bio-industries (Magna Cum Laude)
UCLouvain – September 2013 - June 2015
6-month Erasmus exchange (Wageningen University, the Netherlands) – 2015
Skills
Oral and written scientific communication
- 11 publications in peer-reviewed scientific journals
- 4 talks at international conferences
- Finalist at the UCLouvain contest ‘Ma thèse en 180 secondes’
Management
- Supervision of three Master theses
- 5-day training on team dynamics
Laboratory work
- Cell biology (mammalian, bacterial and yeast cell culture)
- Molecular biology (DNA/RNA extraction, molecular cloning, expression of recombinant proteins, PCR amplification, mutagenesis, RNA sequencing, transfection)
- li>Biochemistry (protein production/purification, Western blot, enzymology, sub-cellular fractionation, use of fluorescent sensors, protein-protein interactions, confocal microscopy)
Teaching
- Supervision of practicals (genetics, cell biology)
UCLouvain – 2018-2019
Languages
French - native language
English - Excellent oral and written fluency
Dutch - Good passive understanding – average oral command
Publications
Thines, L., Roushar, F.J., Hedman, A.C. and Sacks, D.B. (2023). The IQGAP scaffolds: critical nodes bridging receptor activation to cellular signaling. Journal of Cell Biology, 222(6).
Thines, L., Li, Z. and Sacks, D.B. (2023). IQGAP1 is a phosphotyrosine-regulated scaffold for SH2-containing proteins. Cells, 12(3).
Deschamps, A., Thines, L., Colinet, A.S., Stribny, J. and Morsomme, P. (2023). The yeast Gdt1 protein mediates the exchange of H+ for Ca2+ and Mn2+ influencing the Golgi pH. Journal of Biological Chemistry, 299 (5).
Sayedyahossein, S., Smith, J., Barnaeva, E., Li, Z., Choe, C., Ronzetti, M., Dextras, C., Hu, X., Marugan, J., Southall, N., Baljinnyam, B., Thines, L., Tran, A., Ferrer, M. and Sacks, D.B. (2022). Discovery of small molecule inhibitors that effectively disrupt IQGAP1-Cdc42 interaction in breast cancer cells. Scientific Reports, 12(17372).
Thines, L., Gorisse, L., Li, Z., Sayedyahossein, S. and Sacks, D.B. (2022). Calmodulin activates the Hippo pathway by promoting LATS1 kinase-mediated inhibitory phosphorylation of the transcriptional coactivator YAP. Journal of Biological Chemistry, 298(5).
Thines, L., Stribny, J. and Morsomme, P. (2020). From the Uncharacterized Protein Family 0016 to the GDT1 family: Molecular insights into a newly-characterized family of cation secondary transporters. Microbial cell, 7(8).
Stribny, J., Thines, L., Deschamps, A., Goffin, P. and Morsomme, P. (2020). The human Golgi protein TMEM165 transports calcium and manganese in yeast and bacterial cells. Journal of Biological Chemistry, 295(12).
Thines, L., Deschamps, A., Stribny, J. and Morsomme, P. (2019). Yeast as a tool for deeper understanding of human manganese-related diseases. Genes, 10 (545).
Thines, L., Deschamps, A., Sengottaiyan, P., Savel, O., Stribny, J. and Morsomme, P. (2018). The yeast protein Gdt1p transports Mn2+ ions and thereby regulates manganese homeostasis in the Golgi. Journal of Biological Chemistry, 293(21).
Colinet, A.S., Thines, L., Deschamps, A., Flémal, G., Demaegd, D. and Morsomme, P. (2017). Acidic and uncharged polar residues in the consensus motifs of the yeast Ca2+ transporter Gdt1p are required for calcium transport. Cellular Microbiology, 19(7).
Colinet, A.S., Sengottaiyan, P., Deschamps, A., Colsoul, M.L., Thines, L., Demaegd, D., Duchêne, M.C., Foulquier, F., Hols, P. and Morsomme, P. (2016). Yeast Gdt1 is a Golgi-localized calcium transporter required for stress-induced calcium signaling and protein glycosylation. Scientific Reports, 6(1).